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a Schematic illustration of the <t>RNA-seq</t> and metabolomics analyses. b Gene cluster trend in different groups ( n = 3 mice). c GO enrichment analysis of genes in cluster 1. d GO enrichment analysis of genes in cluster 3. e GO enrichment analysis of genes in cluster 4. GO enrichment analysis was performed using a one-sided hypergeo metric test. Terms with a p < 0.05 were considered significantly enriched. f Heatmap o f the relative abundance of proinflammatory cytokines and purine metabolic genes in the three groups ( n = 3 mice). g PCA score plot of different groups subjected to metabolic analysis. h Pathway enrichment analysis of the DEMs enriched in ALI mice treated with ND@Monos (vs. the ALI group). Significance of enrichment was determined by a hypergeometric test with an FDR-corrected p -value ( q -value) threshold of 0.05. i , j Western blot analysis and quantification of IL-1β, P-creb and P-P65 levels ( n = 5 mice). Data are presented as mean ± SD. NC group indicates normal mice, and PBS indicates mice treated with vehicle. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.
Rna Sequencing Transcriptome Library, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+library+and+transcriptome+sequencing/pmc12946169-402-1-16?v=Illumina+Inc
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transcriptome library preparation and rna sequencing  (Macrogen)
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Macrogen transcriptome library preparation and rna sequencing
a Schematic illustration of the <t>RNA-seq</t> and metabolomics analyses. b Gene cluster trend in different groups ( n = 3 mice). c GO enrichment analysis of genes in cluster 1. d GO enrichment analysis of genes in cluster 3. e GO enrichment analysis of genes in cluster 4. GO enrichment analysis was performed using a one-sided hypergeo metric test. Terms with a p < 0.05 were considered significantly enriched. f Heatmap o f the relative abundance of proinflammatory cytokines and purine metabolic genes in the three groups ( n = 3 mice). g PCA score plot of different groups subjected to metabolic analysis. h Pathway enrichment analysis of the DEMs enriched in ALI mice treated with ND@Monos (vs. the ALI group). Significance of enrichment was determined by a hypergeometric test with an FDR-corrected p -value ( q -value) threshold of 0.05. i , j Western blot analysis and quantification of IL-1β, P-creb and P-P65 levels ( n = 5 mice). Data are presented as mean ± SD. NC group indicates normal mice, and PBS indicates mice treated with vehicle. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.
Transcriptome Library Preparation And Rna Sequencing, supplied by Macrogen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+library+and+transcriptome+sequencing/pm40499687-98-9-1?v=Macrogen
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rna library preparation and transcriptome sequencing  (StarSEQ GmbH)
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a Schematic illustration of the <t>RNA-seq</t> and metabolomics analyses. b Gene cluster trend in different groups ( n = 3 mice). c GO enrichment analysis of genes in cluster 1. d GO enrichment analysis of genes in cluster 3. e GO enrichment analysis of genes in cluster 4. GO enrichment analysis was performed using a one-sided hypergeo metric test. Terms with a p < 0.05 were considered significantly enriched. f Heatmap o f the relative abundance of proinflammatory cytokines and purine metabolic genes in the three groups ( n = 3 mice). g PCA score plot of different groups subjected to metabolic analysis. h Pathway enrichment analysis of the DEMs enriched in ALI mice treated with ND@Monos (vs. the ALI group). Significance of enrichment was determined by a hypergeometric test with an FDR-corrected p -value ( q -value) threshold of 0.05. i , j Western blot analysis and quantification of IL-1β, P-creb and P-P65 levels ( n = 5 mice). Data are presented as mean ± SD. NC group indicates normal mice, and PBS indicates mice treated with vehicle. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.
Rna Library Preparation And Transcriptome Sequencing, supplied by StarSEQ GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna library preparation and transcriptome sequencing - by Bioz Stars, 2026-07
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whole transcriptome sequencing  (Illumina Inc)
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Illumina Inc whole transcriptome sequencing
a Schematic illustration of the <t>RNA-seq</t> and metabolomics analyses. b Gene cluster trend in different groups ( n = 3 mice). c GO enrichment analysis of genes in cluster 1. d GO enrichment analysis of genes in cluster 3. e GO enrichment analysis of genes in cluster 4. GO enrichment analysis was performed using a one-sided hypergeo metric test. Terms with a p < 0.05 were considered significantly enriched. f Heatmap o f the relative abundance of proinflammatory cytokines and purine metabolic genes in the three groups ( n = 3 mice). g PCA score plot of different groups subjected to metabolic analysis. h Pathway enrichment analysis of the DEMs enriched in ALI mice treated with ND@Monos (vs. the ALI group). Significance of enrichment was determined by a hypergeometric test with an FDR-corrected p -value ( q -value) threshold of 0.05. i , j Western blot analysis and quantification of IL-1β, P-creb and P-P65 levels ( n = 5 mice). Data are presented as mean ± SD. NC group indicates normal mice, and PBS indicates mice treated with vehicle. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.
Whole Transcriptome Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna library and transcriptome sequencing  (Novogene)
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a Schematic illustration of the <t>RNA-seq</t> and metabolomics analyses. b Gene cluster trend in different groups ( n = 3 mice). c GO enrichment analysis of genes in cluster 1. d GO enrichment analysis of genes in cluster 3. e GO enrichment analysis of genes in cluster 4. GO enrichment analysis was performed using a one-sided hypergeo metric test. Terms with a p < 0.05 were considered significantly enriched. f Heatmap o f the relative abundance of proinflammatory cytokines and purine metabolic genes in the three groups ( n = 3 mice). g PCA score plot of different groups subjected to metabolic analysis. h Pathway enrichment analysis of the DEMs enriched in ALI mice treated with ND@Monos (vs. the ALI group). Significance of enrichment was determined by a hypergeometric test with an FDR-corrected p -value ( q -value) threshold of 0.05. i , j Western blot analysis and quantification of IL-1β, P-creb and P-P65 levels ( n = 5 mice). Data are presented as mean ± SD. NC group indicates normal mice, and PBS indicates mice treated with vehicle. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.
Rna Library And Transcriptome Sequencing, supplied by Novogene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna library preparation, transcriptome sequencing, bioinformatic analysis  (Novogene)
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Novogene rna library preparation, transcriptome sequencing, bioinformatic analysis
a Schematic illustration of the <t>RNA-seq</t> and metabolomics analyses. b Gene cluster trend in different groups ( n = 3 mice). c GO enrichment analysis of genes in cluster 1. d GO enrichment analysis of genes in cluster 3. e GO enrichment analysis of genes in cluster 4. GO enrichment analysis was performed using a one-sided hypergeo metric test. Terms with a p < 0.05 were considered significantly enriched. f Heatmap o f the relative abundance of proinflammatory cytokines and purine metabolic genes in the three groups ( n = 3 mice). g PCA score plot of different groups subjected to metabolic analysis. h Pathway enrichment analysis of the DEMs enriched in ALI mice treated with ND@Monos (vs. the ALI group). Significance of enrichment was determined by a hypergeometric test with an FDR-corrected p -value ( q -value) threshold of 0.05. i , j Western blot analysis and quantification of IL-1β, P-creb and P-P65 levels ( n = 5 mice). Data are presented as mean ± SD. NC group indicates normal mice, and PBS indicates mice treated with vehicle. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.
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rna library preparation, transcriptome sequencing, bioinformatic analysis - by Bioz Stars, 2026-07
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rna library preparation and transcriptome sequencing  (Novogene)
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Novogene rna library preparation and transcriptome sequencing
a Schematic illustration of the <t>RNA-seq</t> and metabolomics analyses. b Gene cluster trend in different groups ( n = 3 mice). c GO enrichment analysis of genes in cluster 1. d GO enrichment analysis of genes in cluster 3. e GO enrichment analysis of genes in cluster 4. GO enrichment analysis was performed using a one-sided hypergeo metric test. Terms with a p < 0.05 were considered significantly enriched. f Heatmap o f the relative abundance of proinflammatory cytokines and purine metabolic genes in the three groups ( n = 3 mice). g PCA score plot of different groups subjected to metabolic analysis. h Pathway enrichment analysis of the DEMs enriched in ALI mice treated with ND@Monos (vs. the ALI group). Significance of enrichment was determined by a hypergeometric test with an FDR-corrected p -value ( q -value) threshold of 0.05. i , j Western blot analysis and quantification of IL-1β, P-creb and P-P65 levels ( n = 5 mice). Data are presented as mean ± SD. NC group indicates normal mice, and PBS indicates mice treated with vehicle. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.
Rna Library Preparation And Transcriptome Sequencing, supplied by Novogene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna-seq libraries for conventional transcriptome sequencing  (Novogene)
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a Schematic illustration of the <t>RNA-seq</t> and metabolomics analyses. b Gene cluster trend in different groups ( n = 3 mice). c GO enrichment analysis of genes in cluster 1. d GO enrichment analysis of genes in cluster 3. e GO enrichment analysis of genes in cluster 4. GO enrichment analysis was performed using a one-sided hypergeo metric test. Terms with a p < 0.05 were considered significantly enriched. f Heatmap o f the relative abundance of proinflammatory cytokines and purine metabolic genes in the three groups ( n = 3 mice). g PCA score plot of different groups subjected to metabolic analysis. h Pathway enrichment analysis of the DEMs enriched in ALI mice treated with ND@Monos (vs. the ALI group). Significance of enrichment was determined by a hypergeometric test with an FDR-corrected p -value ( q -value) threshold of 0.05. i , j Western blot analysis and quantification of IL-1β, P-creb and P-P65 levels ( n = 5 mice). Data are presented as mean ± SD. NC group indicates normal mice, and PBS indicates mice treated with vehicle. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.
Rna Seq Libraries For Conventional Transcriptome Sequencing, supplied by Novogene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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preparation of rna library and transcriptome sequencing  (Novogene)
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a Schematic illustration of the <t>RNA-seq</t> and metabolomics analyses. b Gene cluster trend in different groups ( n = 3 mice). c GO enrichment analysis of genes in cluster 1. d GO enrichment analysis of genes in cluster 3. e GO enrichment analysis of genes in cluster 4. GO enrichment analysis was performed using a one-sided hypergeo metric test. Terms with a p < 0.05 were considered significantly enriched. f Heatmap o f the relative abundance of proinflammatory cytokines and purine metabolic genes in the three groups ( n = 3 mice). g PCA score plot of different groups subjected to metabolic analysis. h Pathway enrichment analysis of the DEMs enriched in ALI mice treated with ND@Monos (vs. the ALI group). Significance of enrichment was determined by a hypergeometric test with an FDR-corrected p -value ( q -value) threshold of 0.05. i , j Western blot analysis and quantification of IL-1β, P-creb and P-P65 levels ( n = 5 mice). Data are presented as mean ± SD. NC group indicates normal mice, and PBS indicates mice treated with vehicle. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.
Preparation Of Rna Library And Transcriptome Sequencing, supplied by Novogene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a Schematic illustration of the RNA-seq and metabolomics analyses. b Gene cluster trend in different groups ( n = 3 mice). c GO enrichment analysis of genes in cluster 1. d GO enrichment analysis of genes in cluster 3. e GO enrichment analysis of genes in cluster 4. GO enrichment analysis was performed using a one-sided hypergeo metric test. Terms with a p < 0.05 were considered significantly enriched. f Heatmap o f the relative abundance of proinflammatory cytokines and purine metabolic genes in the three groups ( n = 3 mice). g PCA score plot of different groups subjected to metabolic analysis. h Pathway enrichment analysis of the DEMs enriched in ALI mice treated with ND@Monos (vs. the ALI group). Significance of enrichment was determined by a hypergeometric test with an FDR-corrected p -value ( q -value) threshold of 0.05. i , j Western blot analysis and quantification of IL-1β, P-creb and P-P65 levels ( n = 5 mice). Data are presented as mean ± SD. NC group indicates normal mice, and PBS indicates mice treated with vehicle. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: A DNA-based nanodevice senses purinergic signaling and drives an immune switch for resolving inflammation

doi: 10.1038/s41467-026-68879-2

Figure Lengend Snippet: a Schematic illustration of the RNA-seq and metabolomics analyses. b Gene cluster trend in different groups ( n = 3 mice). c GO enrichment analysis of genes in cluster 1. d GO enrichment analysis of genes in cluster 3. e GO enrichment analysis of genes in cluster 4. GO enrichment analysis was performed using a one-sided hypergeo metric test. Terms with a p < 0.05 were considered significantly enriched. f Heatmap o f the relative abundance of proinflammatory cytokines and purine metabolic genes in the three groups ( n = 3 mice). g PCA score plot of different groups subjected to metabolic analysis. h Pathway enrichment analysis of the DEMs enriched in ALI mice treated with ND@Monos (vs. the ALI group). Significance of enrichment was determined by a hypergeometric test with an FDR-corrected p -value ( q -value) threshold of 0.05. i , j Western blot analysis and quantification of IL-1β, P-creb and P-P65 levels ( n = 5 mice). Data are presented as mean ± SD. NC group indicates normal mice, and PBS indicates mice treated with vehicle. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.

Article Snippet: The RNA sequencing transcriptome library was generated with a TruSeq TM RNA sample preparation kit from Illumina (San Diego, CA). cDNA was synthesized via a SuperScript double-stranded cDNA synthesis kit (Invitrogen) with random hexamer primers (Illumina).

Techniques: RNA Sequencing, Western Blot, Comparison